Inability to enter S phase and defective RNA polymerase II CTD phosphorylation in mice lacking Mat1
نویسندگان
چکیده
منابع مشابه
Gene-specific RNA polymerase II phosphorylation and the CTD code
Pol II has a unique CTD on its large subunit that comprises heptad repeats (26 in budding yeast, 52 in humans) with a conserved YSPTSPS consensus sequence. This domain serves as a landing pad that presents a dynamic surface to proteins that interact with the transcription elongation complex (TEC) and carry out co-transcriptional pre-mRNA processing and histone modification1. Phosphorylation and...
متن کاملPhosphorylation and functions of the RNA polymerase II CTD.
The C-terminal repeat domain (CTD), an unusual extension appended to the C terminus of the largest subunit of RNA polymerase II, serves as a flexible binding scaffold for numerous nuclear factors; which factors bind is determined by the phosphorylation patterns on the CTD repeats. Changes in phosphorylation patterns, as polymerase transcribes a gene, are thought to orchestrate the association o...
متن کاملRat1p maintains RNA polymerase II CTD phosphorylation balance.
In S. cerevisiae, the 5'-3' exonuclease Rat1p partakes in transcription termination. Although Rat1p-mediated RNA degradation has been suggested to play a role for this activity, the exact mechanisms by which Rat1p helps release RNA polymerase II (RNAPII) from the DNA template are poorly understood. Here we describe a function of Rat1p in regulating phosphorylation levels of the C-terminal domai...
متن کاملBrd4 activates P-TEFb for RNA polymerase II CTD phosphorylation
The bromodomain protein Brd4 regulates the transcription of signal-inducible genes. This is achieved by recruiting the positive transcription elongation factor P-TEFb to promoters by its P-TEFb interaction domain (PID). Here we show that Brd4 stimulates the kinase activity of P-TEFb for phosphorylation of the C-terminal domain (CTD) of RNA polymerase II over basal levels. The CTD phosphorylatio...
متن کاملHeptad-Specific Phosphorylation of RNA Polymerase II CTD.
The carboxy-terminal domain (CTD) of RNA polymerase II (Pol II) consists of heptad repeats with the consensus motif Y1-S2-P3-T4-S5-P6-S7. Dynamic phosphorylation of the CTD coordinates Pol II progression through the transcription cycle. Here, we use genetic and mass spectrometric approaches to directly detect and map phosphosites along the entire CTD. We confirm phosphorylation of CTD residues ...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2001
ISSN: 1460-2075
DOI: 10.1093/emboj/20.11.2844